Abstract: This study aims at phosphate-solubilizing bacteria in maize rhizosphere soil and phosphorus soluble performance under laboratory conditions, in order to provide good bacteria and practical basis for microbial fertilizer development and production. P- solubilizing strains were screened from maize rhizosphere soil samples, the efficient solubilizing strains were optimized by the method of comparison soluble phosphorus circles. The P-solubilizing characteristics of preferred strains were studied by liquid culture conditions of Ca3 (PO4)2, phosphate tailings and soil, the strains were analyzed and identified by 16SrDNA sequence analysis. 19 kinds of phosphorus bacteria were isolated from the soil of maize rhizosphere and an efficient strain named PS- x was obtained by the method of comparison soluble phosphorus circles. The results of P- solubilizing characteristics study showed that PS-1 had strong ability of dissolving Ca3(PO4)2 and phosphate tailings. After 6 days cultivation in the medium with Ca3(PO4)2 as the sole phosphorus source under 35℃ , the soluble phosphorus content reached 429.2 mg/L, which was 45.95 times as much as that of the control group. After 7 days cultivation in the medium with phosphorus tailings powder as the sole phosphorus source, the soluble phosphorus content reached 61.72 mg/L, the phosphate solubilization rate was 21.2%. Available phosphorus content increased 19.28 mg/kg after 50 days cultivation in the soil. The results of sequence analysis of 16SrDNA showed that PS-1 was Bacillus licheniformis. The results showed that P-dissolving bacteria PS-1 had a strong dissolving ability and good application prospect, the growth- promoting effect evaluation and research could be further carried out.