Abstract: In order to alleviate the damage of tomato virus diseases, reduce chemical pesticide consumption and residue, and prevent environmental pollution, the methods of forecasting, randomized block design and statistical analysis were used in field efficacy tests of two biological pesticides of Lentinan Gibberellinheteroauxin-rassinolide (GHB) and Ningnanmycin GHB. The results showed that, when the autumn tomato virus disease was serious, 0.5% Lentinan AS 3000 mL GHB WP 600 g and 8% Ningnanmycin AS 900 mL GHB WP 600 g per hectare were applied for 4 times, once in 7 days, and the control effects on the 10th , 17th , 26th and 35th day were above 88%, 87%, 78% and 67%. The difference between the two biological pesticides was not significant, while the control effect of Lentinan GHB was significantly higher than that of moroxydine hydrochloride (CK) GHB, and the control effect of Ningnanmycin GHB was significantly higher than that of moroxydine hydrochloride GHB. These results indicated that Lentinan GHB and Ningnanmycin GHB were the ideal biological pesticides, which could be used not only for controlling tomato virus diseases, but also for realizing modern, pollution-free, green and organic agricultural production.