Establishment of a Tissue Culture and Rapid Propagation System of Lagerstroemia indica‘Xiaoming 1’

doi:10.11924/j.issn.1000-6850.casb15100075

Abstract

Abstract: In order to establish a tissue culture and rapid propagation system, taking stems with axillary buds of Lagerstroemia indica‘Xiaoming 1’as testing materials, basic medium, concentrations of plant growth regulators, proliferation and rooting stage, and the transplanting method were studied. The results showed that the medium DKW BA 1.0 mg/L NAA 0.05 mg/L PVP 300 mg/L was suitable for inducing germination of stem auxiliary buds, and the rate of germination was up to 95.56%. The best proliferation medium was DKW BA 1.0 mg/L IBA 0.05 mg/L, and the multiplication coefficient was 5.08 with strong plantlets. The rooting percentage, average root number and length per shoot reached 100%, 6.2 and 4.12 cm, respectively, on the medium 1/2DKW NAA 0.1 mg/L AC 300 mg/L. Taking nutrition pot as container, peat soil and perlite as transplanting medium (3:1 or 2:1), the transplant survival rate was over 95.33%. There were many lateral roots and the plantlets which grew rapidly and vigorously. This method was suitable for tissue culture of L. indica ‘Xiaoming 1’and laid foundations for its large-scaled propagation and large area promotion.

CLC Number:

S339.4

References

[1] 方文培. 中国植物志[M].北京:科学出版社, 1983, 52(2):94.
[2] 王晓明, 李永欣, 余格非, 等. 紫薇新品种及繁殖技术[J]. 中国城市林业, 2008,6(1):79-80.
[3] 黄钦才. 紫薇腋芽培养[J]. 植物生理学通讯,1984,33(3) : 44-44.
[4] 姜旭红, 宋刚, 张虎, 等. 日本紫薇的组织培养与快速繁殖[J]. 植物生理学通讯, 2004, 40(6): 707-707.
[5] 宋平. 紫薇再生体系的建立及多倍体诱导研究[D]. 北京:北京林业大学, 2009.
[6] 李国瑞. 紫薇快速繁殖及植株再生的研究[D]. 湖北:华中农业大学, 2010.
[7] 曹受金, 刘辉华, 田英翠. 紫薇的组织培养与快速繁殖[J]. 北方园艺, 2010,(8):149-151.
[8] 杨彦伶, 杨柳, 张亚东. 紫薇组织培养技术. 林业科技开发[J], 2005,19(2):50-52.
[9] 段丽君, 李国瑞, 童俊, 等. 紫薇离体茎段快速繁殖体系研究[J]. 江西农业大学学报, 2013,35(4) : 709-714.
[10] 唐丽丹. 紫薇组织培养快繁研究初探[D].河南:河南农业大学, 2014.
[11] Niranjan M H, Sudarshana M S, Girisha S T. In vitro multiple shoot induction from excised shoot tips and nodal segment explants of - Lagerstroemia indica (L) - A medicinal cum Ornamental Shrub[J]. Journal of Biomedical Sciences and Research, 2012, 2 (3):212-217.
[12] 李晓青, 王慧瑜, 张晓申, 等. 紫薇组培快繁技术研究[J]. 现代农业科技,2009, (19):91-91,93.
[13] 陈磊. 紫薇无性系建立及多倍体诱导技术研究[D]. 天津: 天津大学, 2011.
[14]王轲, 石俊, 鞠易倩, 等. 黑钻石系列紫薇品种组培快繁体系的建立[A].见：张启翔. 中国观赏园艺研究进展[C].北京:中国林业出版社,2014:364-368.
[15] 王晓娇. 紫薇同源四倍体的获得及组培快繁技术研究[D]. 北京:北京林业大学, 2013.
[16] 王闯, 刘敏, 刘殿红, 等. 矮生紫薇的组织培养与再生技术研究[J]. 安徽农业科学, 2010, 38(8):3914 -3915, 3924.
[17] Wang X J, Wang X F, Cai M, et al. In vitro chromosome doubling and tetraploid identification in Lagerstroemia indica[J]. Journal of Food, Agriculture Environment, 2012,10(3,4) :1364-1367.
[18] Zhang Q Y, Luo F X, Liu L, et al. In vitro induction of tetraploids in crape myrtle (Lagerstroemia indica L.) [J]. Plant Cell, Tissue and Organ Culture,2010,101(1):41–47.
[19] 唐兴国, 周全, 范莹. 紫薇幼胚培养及植株再生初探[J].安徽农业科学, 2014, 42( 36): 13177- 13178, 13184.
[20] Paily J, D'Souza L.In vitro clonal propagation of Lagerstroemia flos-reginae Retz[J]. Plant Cell, Tissue and Organ Culture,1986, 6(1): 41-45.
[21] Quraishi A, Koche V, Mishra S K. Micropropagation of Lagerstroemia parviflora Through Axillary Bud Culture[J]. Silvae Genetica,1997,46, (4):242-245.
[22]SVijayan A, Padmesh Pillai P, Hemanthakumar A S, et al. Improved in vitro propagation, genetic stability and analysis of corosolic acid synthesis in regenerants of Lagerstroemia speciosa (L.) Pers. by HPLC and gene expression profiles[J]. Plant Cell, Tissue and Organ Culture,2015,120(3):1209-1214.
[23] 陈怡佳, 崔媛媛, 张晓明, 等. 美国红叶紫薇的组织培养与快速繁殖[J]. 植物生理学报,2015, 51 (6): 882-886.
[24] 王丹, 柴慈江, 骆建霞, 等. 外源激素对矮生紫薇组培快繁的影响[J]. 北方园艺,2009,(12):194-196.
[25] Eymar E, Alegre J, Toribio M, et al. Effect of activated charcoal and 6-benzyladenine on in vitro nitrogen uptake by Lagerstroemia indica[J]. Plant Cell, Tissue and Organ Culture, 2000,63(1): 57–65.