Abstract: The objective of this study is to figure out the effects of bast fiber film on rice seedling growth. In this study, indica cultivar ‘Xiangwanxian 13’ and indica hybrid cultivar ‘Y liangyou 1’ were used to investigate the changes of aboveground part growth and root respiration enzyme activities of rice seedlings treated with bast fiber film. The results showed that, compared with the control, the accumulation of shoot biomass, chlorophyll content, root bleeding sap, soluble sugar in shoot, and plant NO₃-N content were significantly enhanced after bast fiber film treatment. In addition, the activities of malate dehydrogenas (MDH) and alcohol dehydrogenas (ADH) were higher in rice seedling roots treated with bast fiber film, while there was no significant change of lactate dehydrogenase (LDH) after bast fiber film treatment. However, there was no significant difference of these indicators between the two cultivars. All the results indicated that the growth and development of rice seedlings could be improved by the application of bast fiber film.