Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (14): 17-20.doi: 10.11924/j.issn.1000-6850.casb15120143

Special Issue: 畜牧兽医

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Isolation and Characterization of Escherichia coli Strain from Egg-laying Chickens with Salpingitis

Zhu Chunhua, Huang Yu, Liu Binqiong, Chen Zhen, Chen Cuiteng   

  1. (Animal Husbandry and Veterinary Medicine Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013)
  • Received:2015-12-24 Revised:2016-05-04 Accepted:2016-03-22 Online:2016-05-17 Published:2016-05-17

Abstract: A bacterial strain (FQ-180) was isolated from one layer flock in Fujian in 2015. To study its biological characteristics, after preliminary purification, Gram′s staining and electron microscope observation, the strain was primarily identified to be Escherichia coli. The antigen analysis showed that the serotype of the strain was O78. The strain showed highly sensitivity to four kinds of common antibiotics including amoxicillin, florfenicol, cefalexin and cefazolin. Specified genomic 16S rDNA was amplified by PCR, and the segment length was about 1470 bp, which indicated that the isolated strain was Escherichia coli.