Abstract: To investigate the changes in the expression of GlyCAM-1 in uterus of oestrous sheep which was artificially induced, expression changes of GlyCAM-1 on the 10^th, 12^th, 14^th, 16^th and 18^th day after sheep oestrus were detected respectively by Real-Time PCR and Western blot. The results of Real-Time PCR showed that mRNA of sheep GlyCAM-1 increased on the 10^th day, decreased on the 14^th and 16^th day, but increased again on the 18^th day, which were in accordance with the results of protein expression detected by Western blot. Protein of 34 kD was detected by this method with specific antiserum. The transcriptional and expressional level of GlyCAM-1 on the 10^th day was significantly higher than that of the other days (P<0.05). In addition, GlyCAM-1 mRNA expression on the 12^th day reduced significantly (P<0.05), and then remained relatively constant, but significantly increased on the 18^th day. There were no significant difference of protein expression among the 12^th, 14^th and 16^th day (P>0.05), but there were significant differences among the other days (P<0.05). Protein expression of the 18^th day increased significantly (P<0.05). Thus it could be concluded that during the 10^th-18^th day after sheep oestrus, the transcriptional and expressional level of GlyCAM-1 presented a decrease-increase trend.