Abstract: In order to explore genetic effects and heterosis expression of two-line-method Brassica napus L.hybridized combination in different ecological environments, the authors used 6 rapeseed restorer lines of different nuclear backgrounds as parents to make 36 cross combinations according to an incomplete diallel design (NC II). These cross combinations were planted in two different ecological areas Dali and Zhangye, the traits were investigated in mature period and ADE model of Zhujun was used to analyze these data. The results showed that yield traits were influenced by additive effect, dominance effect, and the environment interaction effect in different degrees, the pod numbers per plant and thousand seed weight were mainly influenced by additive effect, yield per plant and per pod grain number were mainly influenced by dominance effect. In environment interaction effect, all genetic effect of yield per plant, pod numbers per plant and thousand seed weight achieved significance with environment interactions, but the hereditary of per pod grain number was relatively stable. In genotype and environment interaction effect, the effective branch height and first effective branch number were affected by environmental effects. As to the genetic correlation, phenotype correlation coefficient and genotype correlation coefficient of yield per plant and pod numbers per plant, per pod grain number and thousand seed weight all reached the significant level. Heterosis of hybridized combination overall performance showed that F₁ generation was better than F₂ generation. The results give significant guidance for breeding two-line hybrid combinations.