Abstract: In order to reveal the relationship between Lepidium meyenii root weight and main agronomic characters, and provide references for high yield cultivation of Lepidium meyenii, field performance of the same strain in 11 regions was studied through the methods of correlation, regression balance and path coefficient analysis. The results showed that fresh root weight (y) and coverage area (x1), stem diameter (x2), enlargement coarse (x4) and broad leaves (x12) were highly and significantly correlated. The optimal regression equation was y=- 24.584 0.003x1- 10.313x2 18.011x4- 0.738x5- 0.957x6 0.964x7 1.192x10- 1.803x11 9.14x12. The enlargement root thickness, broad leaves, coverage area, root number and petiole length had positive path coefficients for the fresh root weight. Main root length, leaf length, stem diameter and enlargement root length had negative path coefficients for the fresh root weight. In the cultivation and management process, to realize the increase of root fresh weight, the cultivation should be focused on the enlargement root thickness of the underground part and the leaf width of the aboveground part.