Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (26): 27-32.doi: 10.11924/j.issn.1000-6850.casb16030116

Special Issue: 生物技术 园艺

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Inhibition Capability of Camellia Pollen Extract on α-Amylase

Wu Zhonggao1, Ni Hui2, Yang Yuanfan2, Wang Xing1   

  1. (1Beijing Sericulture and Apiculture Management Station, Beijing 100029;2College of Food and Biological Engineering, Jimei University, Xiamen Fujian 361021)
  • Received:2016-03-15 Revised:2016-04-22 Accepted:2016-05-31 Online:2016-09-21 Published:2016-09-21

Abstract: Bee pollen is a kind of bee products that has wide resources and lots of important physiological functions. In this paper, Camellia pollen extract (CPE) prepared by ethanol extraction was investigated for its α-amlyase inhibitory activity. As the results, the pollen extract significantly inhibited the activity of α-amlyase. The half-inhibitory concentration (IC50) was determined to be 8.94 mg/mL. The inhibition belonged to non-competitive reversible inhibition with an inhibition constant (Ki) of 1.31 mg/mL. Lutin and gallic acid respectively affected the inhibitory activity as the positive and negative synergist, however, their concentrations did not display consistent trends on the synergistic rate. The strong synergistic effect of vitamin C on the inhibition of CPE was a negative model, which increased along with the increase of vitamin C concentration. These results provide a theoretical reference for comprehensive utilization of bee pollen and the development of α-amylase inhibitors from nature products.

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