Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (24): 126-129.doi: 10.11924/j.issn.1000-6850.casb16030160

Previous Articles     Next Articles

Extraction of Monocrotaline from Crotalaria pallida Seed

Zhang Xuyuan, Luo Haixi, Yu Yuling, Pei Donggui   

  1. (School of Biological and Chemical Industry, Hainan Institute of Science and Technology, Haikou 571126)
  • Received:2016-03-21 Revised:2016-06-24 Accepted:2016-06-24 Online:2016-08-29 Published:2016-08-29

Abstract: In order to improve the extraction yield of monocrotaline and provide wide resources for monocrotaline medicine, monocrotaline was extracted from Crotalaria pallida seeds in Hainan Province. Constant temperature extraction was combined with ultrasonic treatment process, and hydrochloric acid, ethanol, chloroform were used as extraction solvent for the extraction. The crude extract was purified using the dissolution characteristic of the alkaloids. The results showed that the optimal extraction process was using ethanol as extraction solvent with material liquid ratio being 1:4, leaching under 75℃ for 4 hours first, and then using ultrasonic cell crushing apparatus for 30 minutes. Under the above conditions, the average content of monocrotaline from Crotalaria pallida seed was 34.736 mg/g.

CLC Number: