Abstract: In order to reveal the degradation mechanism of AM on atrazine, the new cross-compartments cultivation systems (T type) were built. Medicago sativa which was sensitivity to atrazine was chosen to inform symbiotic association with Glomus mosseae as the research objective. Then the degradation rate of atrazine and soil enzymes activities in rhizosphere were investigated in this study. When atrazine addition level was 20 mg/kg, M. sativa root colonization reached 85.70% in 25 d, and the degradation rate of atrazine was 70.22%, which was attributed to arbuscular mycorrhiza by 77.28%. It was suggested that alfalfa mycorrhiza had high degradation ability on atrazine in soil. Meanwhile, atrazine could promote the activities of saccharase, urease, catalase and cellulase in rhizosphere soil. Under the dual function of G. mosseae and atrazine treatments, these four enzymes activities were further enhanced. In conclusion, this research provided theoretical foundation for AM application to remedy pesticide polluted soil.