PGE2 Can Rescue Normal Development of in vitro Bovine Early Embryos Lacking Endogenous PGs

doi:10.11924/j.issn.1000-6850.casb16040082

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (32): 1-6.doi: 10.11924/j.issn.1000-6850.casb16040082

Special Issue: 畜牧兽医

PGE2 Can Rescue Normal Development of in vitro Bovine Early Embryos Lacking Endogenous PGs

Qin Guosong^1,2, Ni Hemin¹, Liu Yunhai¹, Li Hejuan¹, Sheng Xihui¹, Qi Xiaolong¹, Guo Yong¹

(¹College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206;²State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101)

Received:2016-04-13 Revised:2016-11-01 Accepted:2016-06-24 Online:2016-11-15 Published:2016-11-15

Abstract

Abstract: The study aimed to investigate the effect of exogenous prostaglandin E2 (PGE2) on early embryo in vitro development of bovine IVF which lacked endogenous prostaglandins (PGs). Specific inhibitors of prostaglandins were also added into the culture medium of bovine embryos to inhibit the initiation of these embryonic endogenous prostaglandins, and then, an investigation was conducted to detect the function, the appropriate concentration and work stage of PGE2 on early bovine embryos’ development. In addition, PGE2 specific receptors’ mRNA level was also respectively detected. It was found that bovine IVF embryonic developmental potentialities could decrease the cleavage rate, 8-16 cell rate, blastocyst rate and hatching blastocyst rate significantly compared with those of the control after those embryos’ endogenous PGs being thoroughly inhibited. Then, it was also found that adding exogenous PGE2 could rescue the damage of IVF embryos lacking endogenous PGE2 affected by the adverse effect of PGE2 specific inhibitors. The suitable PGE2 additive concentration was 1×10^-8 mol/L, and its compensation effect occurred before 8-cell stage of those embryos cultured in vitro. However, the mRNA of PGE2’s specific receptors: EP1, EP2, EP3 and EP4 were all not detectable by semi-quantitative RT-PCR. PGE2 could rescue the normal development of those in vitro produced bovine early embryos lacking endogenous PGs.

Qin Guosong,Ni Hemin,Liu Yunhai,Li Hejuan,Sheng Xihui,Qi Xiaolong and Guo Yong. PGE2 Can Rescue Normal Development of in vitro Bovine Early Embryos Lacking Endogenous PGs[J]. Chinese Agricultural Science Bulletin, 2016, 32(32): 1-6.

References

[1] Kowalewski M P, Ihle S, Siemieniuch M J, et al.,Formation of the early canine CL and the role of prostaglandin E2 (PGE2) in regulation of its function: an in vivo approach[J]. Theriogenology, 2015,83(6):1038-47.
[2] McCarthy M K, Procario M C, Wilke C A, et al. Prostaglandin E2 Production and T Cell Function in Mouse Adenovirus Type 1 Infection following Allogeneic Bone Marrow Transplantation[J]. PLoS One, 2015,10(9):e0139235.
[3] Chen J H, Perry C J, Tsui Y C, et al. Prostaglandin E2 and programmed cell death 1 signaling coordinately impair CTL function and survival during chronic viral infection[J]. Nat Med, 2015,21(4):327-34.
[4] Schlagenhauf A, ?Haidl H, ?Leschnik B, et al. Prostaglandin E2 levels and platelet function are different in cord blood compared to adults[J]. Thromb Haemost, 2015,113(1):97-106.
[5] Johansson J U, Woodling N S,?Wang Q, et al. Prostaglandin signaling suppresses beneficial microglial function in Alzheimer''s disease models[J]. J Clin Invest, 2015,125(1):350-364.
[6] Lim H,?Paria B C,?Das S K,et al. Multiple female reproductive failures in cyclooxygenase 2-deficient mice[J]. Cell, 1997,91(2):197-208.
[7] Zhang M, Wang F,?Chong Y, et al. Liver myofibroblasts from hepatitis B related liver failure patients may regulate natural killer cell function via PGE2[J]. J Transl Med, 2014,12:308.
[8] Sugimoto Y, Inazumi T, Tsuchiya S. Roles of prostaglandin receptors in female reproduction[J]. J Biochem, 2015,157(2):73-80.
[9] Rossitto M,?Ujjan S, Poulat F, et al. Multiple roles of the prostaglandin D2 signaling pathway in reproduction[J]. Reproduction, 2015,149(1):R49-58.
[10] Kiraly A J, Soliman E,?Jenkins A, et al. Apigenin inhibits COX-2, PGE2, and EP1 and also initiates terminal differentiation in the epidermis of tumor bearing mice[J]. Prostaglandins Leukot Essent Fatty Acids, 2016,104:44-53.
[11] Zhang M, Feigenson M, Sheu T J, et al. Loss of the PGE2 receptor EP1 enhances bone acquisition, which protects against age and ovariectomy-induced impairments in bone strength[J]. Bone, 2015,72:92-100.
[12] Yang Z M, Das S K,?Wang J, et al. Potential sites of prostaglandin actions in the periimplantation mouse uterus: differential expression and regulation of prostaglandin receptor genes[J]. Biol Reprod, 1997,56(2): 368-379.
[13] Reitmair A, ?Lambrecht N W, Yakubov I, et al. Prostaglandin E2 receptor subtype EP2- and EP4-regulated gene expression profiling in human ciliary smooth muscle cells[J].Physiol Genomics, 2010,42(3):348-360.
[14] Matsumoto H, Ma W,?Smalley W, et al. Diversification of cyclooxygenase-2-derived prostaglandins in ovulation and implantation[J]. Biol Reprod, 2001,64(5):1557-1565.
[15] Ginther O J,?Castro T,?Baldrighi J M, et al. Defective secretion of Prostaglandin F2alpha during development of idiopathic persistent corpus luteum in mares[J]. Domest Anim Endocrinol, 2016,55:60-65.
[16] Pakrasi P L, Dey S K. Blastocyst is the source of prostaglandins in the implantation site in the rabbit[J].Prostaglandins, 1982,24(1):73-77.
[17] Parra T, Benites J,?Ruiz L M, et al. Gastroprotective activity of ent-beyerene derivatives in mice: Effects on gastric secretion, endogenous prostaglandins and non-protein sulfhydryls[J]. Bioorg Med Chem Lett, 2015,25(14):2813-2817.
[18] Biggers J D, Leonov B V, Baskar J F, et al. Inhibition of hatching of mouse blastocysts in vitro by prostaglandin antagonists[J]. Biol Reprod, 1978,19(3):519-533.
[19] Knepper P A, Mayanil C S, Goossens W, et al. The presence of transcription factors in fetal bovine sera[J]. In Vitro Cell Dev Biol Anim, 1998,34(2):170-173.
[20] Scenna F N, ?Edwards J L,?Rohrbach N R,et al. Detrimental effects of prostaglandin F2alpha on preimplantation bovine embryos[J]. Prostaglandins Other Lipid Mediat, 2004,73(3-4):215-26.
[21] Biggers J D, Bell J E, Benos D J. Mammalian blastocyst: transport functions in a developing epithelium[J]. Am J Physiol, 1988,255(4 Pt 1):C419-32.
[22] 刘凤军,王勇胜. 培养液及血清浓度对山羊孤雌胚胎体外培养的影响[J]. 黑龙江畜牧兽医,2004,2(2):11-13.

PGE2 Can Rescue Normal Development of in vitro Bovine Early Embryos Lacking Endogenous PGs

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