Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (32): 15-19.doi: 10.11924/j.issn.1000-6850.casb16040169

Special Issue: 畜牧兽医

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Study on the differential expression of TEKT4 and TEKT5 in Hyline Brown semen before and after freezing

  

  • Received:2016-04-27 Revised:2016-11-01 Accepted:2016-07-25 Online:2016-11-15 Published:2016-11-15

Abstract: Abstract: In this research, the biological function of TEKT4 and TEKT5 genes in the process of chicken semen freezing was studied, laying a theoretical foundation for improving the effect of chicken semen cryopreservation in poultry industry in the future. The semen samples were collected from 30 Hyline Brown Roosters randomly selected and detected for quality. Then, the RT-PCR was applied to detect the transcriptional differences of TEKT4 and TEKT5 before and after semen freezing. The results showed that the transcriptional level of TEKT4 and TEKT5 in cock sperm was significantly decreased after freezing and the fold change was 0.3481 and 0.5741 respectively. Thus, we conclude that TEKT4 and TEKT5 are related to the anti-freezing characteristics of cock semen, and could be studied as a candidate gene in the future.