Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (34): 143-148.doi: 10.11924/j.issn.1000-6850.casb16050088

Special Issue: 资源与环境 园艺

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Genetic Diversity of 21 Kinds of Grape Germplasm Resources by SSR Markers

Liu Wei, Wang Yunyun, Zhao Wujuan, Zhao Xuehui, Dong Zhigang   

  1. (Shanxi Academy of Agricultural Sciences Pomology Institute, Shanxi Key Laboratory of Germplasm Improvement and Utilization in Pomology, Taiyuan 030031)
  • Received:2016-05-16 Revised:2016-11-23 Accepted:2016-07-25 Online:2016-12-15 Published:2016-12-15

Abstract: This study aims to analyze genetic relationship and diversity of various types of grape germplasm resources by SSR molecular marker and provide theoretical basis for resources classification and cultivar identification. 15 pairs of SSR primer were used for PCR amplification in 21 kinds of grape germplasms. The situation of amplification, the genetic distance and relationship between different materials were analyzed. 15 pairs of SSR primer amplified 91 gene loci; including 89 polymorphic loci, the rate of polymorphism was as high as 97.8%. The rate of polymorphism of 13 pairs of SSR primer achieved 100%. The genetic similarity coefficient between the 21 materials ranged from 0.45 to 0.91, and all germplasms were divided into two groups in the genetic similarity coefficient of 0.62. This study showed that SSR molecular marker analyzed the genetic diversity of different kinds of grape germplasms from the molecular level, revealed the relationship between different materials, and provided certain bases for breeding research.