Abstract: In order to study the effect of phosphorus supply on growth and nutrient uptake of tomato (Lycopersicon esculentum Mill), four phosphorus application levels (0, 0.165, 0.660 and 3.300 mmol/L H2PO4-) were conducted in this study by water planting. The results indicated that the tomato biomass was affected by phosphorus deficiency, low phosphorus and high phosphorus; POD, MDA and relative conductivity of leaf were significantly increased in phosphorus deficiency treatment, and the plant was small and thin, the leaf color changed to purple, which showed obvious phosphorus deficiency symptom. The P content in root, stem and leaf of tomato increased with the increase of phosphorus application level, low phosphorus increased the transfer ratio of phosphorus to the ground, and high phosphorus increased P accumulation in the root; K and Ca uptake of tomato were significantly reduced in low phosphorus treatment, the transportation of root K, Ca and Mg to ground was affected by high phosphorus treatment.