The Influence of Boric Acid on Physiology and Uranium Accumulation in Phaseolus vulgaris L. under Uranium Stress

doi:10.11924/j.issn.1000-6850.casb16060149

Abstract

Abstract: The aim is to study the effect of exogenous boric acid on physiological and biochemical indicators of Phaseolus vulgaris L. and uranium enriched capability under uranium stress. We used Phaseolus vulgaris L. as material, carried out the pot controlled experiments by applying 0, 10, 20, 30, 40, 50 mg/kg boric acid in the uranium contaminated soil. Phaseolus vulgaris L. was planted in the greenhouse, and was sampled before flowering, and the physiological indicators and uranium enrichment content were measured. The results showed that: as the boric acid concentration increased, the maximum photochemical efficiency (Fv/Fm), performance index (PIABS), the energy absorption per active reaction center (ABS/RC), the capture capability per active reaction center (TRo/RC), the capture energy used for electron transfer peractive reaction center (ETo/RC) and the non-photochemical energy dissipation per active reaction center (DIo/RC) showed a downward trend after a rise first. Chlorophyll, superoxide dismutase (SOD), peroxidase (POD) activity, soluble protein, soluble sugar, free proline also rose firstly, then declined; Malondialdehyde (MDA) firstly decreased then increased. With exogenous boric acid concentration increased, the uranium in above- ground and under- ground part of Phaseolus vulgaris L. were increased, indicating that appropriate concentration of exogenous boric acid could increase resistance to heavy metals and uranium, as well as the enrichment capacity and the ability to transfer in Phaseolus vulgaris L..

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