Abstract: This study aims at providing references for degrading hyperaccumulation plants by researching cellulase production conditions of a capable of degrading hyperaccumulator Bacillus subtilis subspecies BSC3. By setting different carbon sources, nitrogen sources, inoculation amount, culture temperature, liquid volume, initial pH value and culture time of growing conditions, single factor and orthogonal experiment were carried out. The experiment results showed that the optimum condition for producing cellulase by strain BS-C3 was 1% CMC-Na as carbon source, 1% peptone and 1% yeast powder as nitrogen source, inoculation quantity was 3%, temperature was 37℃, liquid volume was 70 mL, the initial pH value was 8, the filter paper enzyme activity FPA and carboxymethyl cellulase activity CMCA reached the maximum value of 223.74 U/mL and 257.67 U/mL on the fourth day and the fifth day respectively. We obtained the best condition of strain BS-C3 producing cellulase.