Abstract: The nitrogen release characteristics of controlled release urea (L120d and S80d) whose shape of release curve was parabolic (L) and sigmoid (S) and release period was 120 d and 80 d respectively were studied by constant temperature incubation experiment, the release rates (k) were obtained according to SUGIHARA and LOGISTIC models. On the basis of facility tomato experiment, a field experiment was carried out to study the characteristic of nitrogen release and its effect on tomato. The results showed that the k values of L120d and S80d were significantly affected by temperature under indoor culture conditions, k values were 0.0022~0.0212/d and 0.043~0.279/d respectively under 4 kinds of temperature conditions, the release curve showed the characteristics of L and S types respectively. The characteristics of L120d in field experiment was consistent with indoor culture conditions, but the release period of S80d in field condition had the difference of more than 30 d, the nitrogen release characteristic of L120d and S80d was different. Compared with urea basal application and 3 topdressings, tomato yield of L120d and S80d increased by 7.8%, 7.2% and 5.4%, 4.8% in comparing with that of common urea (N 200 kg/hm2) and conventional N rate (N 400 kg/hm2); nitrogen use efficiency (NUE) increased by 5.5-10.1 percentage point, and the economic benefits increased by 22817 and 13375 yuan/hm2. This study provided a scientific basis for the popularization and application of controlledrelease fertilizer in facility agriculture.