Abstract: To further study the regulation of aquaporin expression and the mechanism in plants, we cloned an aquaporin gene MaSIP2- 2 from banana, meanwhile analyzed the gene sequence by the biology software and constructed the plant expression vector. Sequence analysis indicated that MaSIP2-2 had complete ORF, 780 bp, and it encoded 260 amino acids. Multiple sequence alignment and the phylogenetic tree analysis indicated that the protein encoded by MaSIP2- 2 was in high similarity with SIP-encoding protein in the other plants. The homology with amino acid sequences in Musa acuminate, Elaeis guineensis, Citrus sinensis, Phoenix dactylifera, Prunus mume, Theobroma cacao and Ananas comosus was 98%, 62%, 53%, 52%, 57%, 55%, 59%, respectively. The protein molecular weight of MaSIP2-2 was 28800.62 Da, and the theoretical isoelectric point (pI) was 9.13. The hydrophilic amino acids uniformly distributed throughout the peptide, and more than the hydrophobic amino acids. The expression vector of the gene was successfully constructed by PCR and identified by restriction enzyme digestion reactions. The research results showed that MaSIP2-2 was a member of the aquaporin small and basic intrinsic proteins, and provided base for functional verification in the future.