Chinese Agricultural Science Bulletin ›› 2017, Vol. 33 ›› Issue (9): 37-40.doi: 10.11924/j.issn.1000-6850.casb16080106
Special Issue: 园艺
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Abstract: The aim is to select SSR primers for identifying the purity and reality of melon hybrid. Eighteen high- polymorphic SSR (Simple Sequence Repeats) primers were used for polymorphic screening between ‘Zhongtian cuixue’and the parents, and the verification was conducted in cross population. Thirteen SSR primers produced polymorphic bands between two parents. After repeats, we screened two primers which had polymorphism among parents, had polymorphic bands of female and male parent in the hybrid. The results of SSR identification in hybrids were the same as morphological identification in field. RF151 and RF144 could be used as the specific primer to identify the purity of hybrid-‘Zhongtian cuixue’. Because SSR markers have co-dominance traits, SSR is an important method for purity identification of hybrid.
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.casb16080106
https://www.casb.org.cn/EN/Y2017/V33/I9/37