Abstract: Compared with three-line hybrid rice, two-line rice has the advantage of higher breeding efficiency, lower cost of seed production, higher yields, relative superior quality, and so on. In recent years, varieties registration and application of two-line rice was developed rapidly in Yangtze River basin represented by Chongqing. In order to explicit the condition and tendency of two-line rice industry, this article analyze the acreage, main varieties and varieties certification of two-line rice during the year of 2006-2015. The results shows that, the acreage for two-line rice increased rapidly in recent 10 years, covered 17.2% of total acreages in 2014, and ‘Yliangyou No 1’and ‘Shenliangyou5814’cover more acreage than other varieties. On the variety application, the dominant variety rate of two-line rice rose from 5.9% in 2006 to 31.3% in 2005, and all two-line varieties were good quality rice. On the variety evaluation, the certified or recognized varieties were sporadic during 10 years, and almost all varieties was selected by others agricultural scientific research units, varieties evaluation that breeding by Chongqing agricultural research unites has been maintained a record of zero until 2015.