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Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (34): 119-122.doi: 10.11924/j.issn.1000-6850.casb16080126

Special Issue: 园艺

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Establishment and Optimization of SCoT-PCR System in Sugarbeet

Liu Naixin1,2,3, Guo Chenchen1,3, Sun Yanhong1,3, Yu Hongying1,3, Ma Longbiao1,2,3, Wu Zedong1,2,3   

  1. (1Key Laboratory of Sugar Beet Genetic Breeding/Heilongjiang University, Harbin 150080; 2Sugar Beet Research Institute of Chinese Academy of Agricultural Sciences/Crop Academy of Heilongjiang University, Harbin 150080; 3Key Laboratory of North Sugar Crop Resource and Utilization, Chinese Academy of Agricultural Sciences, Harbin 150080)
  • Received:2016-08-26 Revised:2016-10-17 Accepted:2016-10-25 Online:2016-12-15 Published:2016-12-15

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Abstract: The objective of the study is to provide technical support for fingerprint construction, genetic diversity analysis and other molecular marker assisted breeding of sugarbeet, with SCoT molecular markers. This sugarbeet SCoT-PCR reaction system was optimized by using the single factor experiment. The results showed that the SCoT-PCR optimal reaction system contained 2.0 μL of 10×PCR buffer (including Mg2 ), 10 ng of DNA template , 0.5 U of Taq polymerase, 2 μL of 10 μmol/L SCoT primers and 0.1μL of dNTPs (2.5 mmol/L each) in the 20 μL reaction system. Then, 12 varieties of sugarbeets were amplified using the optimized reaction system. The results demonstrated that this system was stable and had clear amplified band, so it could be used for constructing fingerprint of sugarbeet and other molecular biology research.