Abstract: Farmers'' professional cooperative is an effective relationship between production and operation, its external development environment is improving, and its participation in e-commerce has become a necessary way of self- transformation and upgrading. Based on investigation of farmers'' professional cooperatives in Zhejiang Province, this paper used binary logistic model to build a structural equation model, and analyzed the influence factors of the cooperatives’e-commerce development. The results showed that human resource is the main factor affecting the willingness of farmers'' professional cooperatives to participate in e-commerce. The ecommerce of farmers’professional cooperatives could be enhanced by measures such as increasing capital investment, moderate scale operation, upgrading the technical training level of the cooperative members and establishing a platform for experience exchange. In addition, this paper also provided suggestions on the effective participation of farmers’professional cooperatives in e-commerce and the sustainable development ofthis business mode.