Abstract: To explore the effects of deep intra-uterine insemination (DUI) on sow productive performance, 339 multiparous Large White sows were used as research objects. Intra-uterine insemination (IUI) and DUI were used as the main mating manners. The effects of season, semen volume, semen type, frozen semen and sow synchronous estrus on the efficiency of DUI were compared and analyzed for the optimization of semen volume, insemination condition and semen type in the process of mating. The results showed that litter size, and numbers of alive and healthy piglets in DUI treatment were higher than those in IUI treatment both in summer and winter. The indicators were the best when 40 mL semen was used for DUI. When the same amount of semen was inseminated, litter size and number of alive piglets in IUI treatment were significantly lower than those in DUI treatment. However, different semen types did not show significant differences in all the indicators. Pregnancy rate, litter size and number of alive piglets in the treatment of estrus synchronization with fresh semen insemination were significantly higher than those of estrous non-synchronization with fresh or frozen semen insemination. Moreover, litter size, and numbers of alive and weaker piglets were not significantly different in the latter two treatments. In conclusion, DUI can improve sow reproductive performance and increase economic benefits of pig farms to some extent.