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Chinese Agricultural Science Bulletin ›› 2017, Vol. 33 ›› Issue (16): 146-151.doi: 10.11924/j.issn.1000-6850.casb16100125

Special Issue: 园艺

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Polysaccharide Purification from Wudang Taoism Tea by Resin

  

  • Received:2016-10-31 Revised:2017-06-03 Accepted:2016-12-14 Online:2017-06-08 Published:2017-06-08

Abstract: The paper aims to study the effects of resin on decolorization and deproteinization of Wudang Taoism tea, and the effect of DEAE-52 cellulose column chromatography on polysaccharide isolation from Wudang Taoism Tea. Based on single-factor experiments, orthogonal tests were employed to evaluate three factors including sample loading flow rate, temperature and ratio of material to water according to L9(34) scheme. The anthrone-sulfuric acid method and the coomassie brilliant blue method were eparately applied for the content detection of polysaccharide and protein. When the sample was absorbed by 201x7 in the condition of 50℃ , 1.0 mL/min and the ratio (material to water) of 11:1, the rates of decolorization, deproteinization and polysaccharide retention was 87.49% , 43.27% and 89.12% , respectively. Five polysaccharide fractions were isolated by subsequent DEAE-52 cellulose column chromatography. The scheme is steady and effective, and suitable for further development and utilization of olysaccharide from Wudang Taoism tea.