Abstract: The purpose of this study was to evaluate the physiological response characteristics and drought resistance of hybrid millet variety ‘Zhangzagu 3’ under the drought stress condition simulated by PEG.The results will expand the way of agricultureal development in Chinese arid areas and provide support for the hybrid millet cultivation generalization in agricultural production system of rain-fed area. The physiological and biochemistry indexes, such as leaf chlorophyll, free proline, malondialdehyde (MDA) and superoxide dismutase (SOD) , were compared in four cultivars of ‘Zhangzagu 3’, Parental 1, Parental 2 and conventional millet variety ‘8311’ under the different PEG-6000 solution concentrations. Chlorophyll contents of leave in four millet varieties were significantly decreased under the simulated drought conditions with different PEG osmotic stress concentrations and ‘Zhangzagu 3’ was the smallest. Free proline content of leave in four varieties seedling were sharply increased with stress level. MDA contents in leave of the four millet varieties increased with the drought stress aggravation and ‘Zhangzagu 3’ was the smallest. Activities of SOD in four kind seedlings were increased with worsening drought and the activities of SOD under 20% and 30% PEG-6000 concentration were rapidly enhanced and ‘Zhangzagu 3’ was the largest increase varity. The adaptabilities of four tested materials were different under drought stress. For drought tolerance ‘Zhangzagu 3’ was the strongest, ‘8311’ the second, and the Parental 2 the last.