Isolation and Identification of Neurotrophic Factors from Water-soluble Constituents of Carya cathayensis Sarg.

doi:10.11924/j.issn.1000-6850.casb17040076

Chinese Agricultural Science Bulletin ›› 2018, Vol. 34 ›› Issue (15): 159-164.doi: 10.11924/j.issn.1000-6850.casb17040076

Special Issue: 园艺

Received:2017-04-12 Revised:2017-06-08 Accepted:2017-06-23 Online:2018-05-29 Published:2018-05-29

Abstract

Abstract: [Objective] To study the neurotrophic activity of water-soluble constituents from hickory and identify functional factors of it. [Methods] The SH-SY5Y cell was treated with a non-fat hickory water-soluble extract, outgrowth of neurites was detected by microscope and expression of related genes was measured by qPCR method, isolation and identify of neurotrophic factor was carried by HPLC and LC-MS method. [Results] The results showed that hickory water-soluble extract could promote the neurites outgrowth of SH-SY5Y significantly, as well as the gene expression of NF160. The results of HPLC and LC-MS showed that the main constituents of hickory water-soluble are cytoside, urea, guanosine, adenine and adenosine. [Conclusion] It is concluded that hickory water-soluble constituents have neurotrophic activity by manipulate the gene expression of NF160.

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