Abstract: To establish a beet DAMD amplification system and apply DAMD primer in construction of beet varieties fingerprint and molecular marker assisted breeding, this experiment optimized the beet DAMD system by using the single factor variable method. 12 beet varieties were chosen; the optimized system was used to amplify 25 DAMD primers. The optimum DAMD system of beet was as following: a total volume of 20 μL, template DNA 10-80 ng, 0.75 U of DNA polymerase, 0.2 μL of dNTPs (2.5 mmol/L each) and 2.0 μL of primers (10 μmol/L). At the same time, 25 primers were all amplified out of the clear bands, and except individual primers whose polymorphism was poorer, the other primers had very abundant polymorphism. The primers 62 H(-) could distinguish all 12 beet varieties. Thus, DAMD primers had high amplification efficiency, the amplification result was stable and the bands were clear, so DAMD primers were very suitable for the construction of fingerprint and the genetic diversity analysis of beet.