Abstract: The objective is to analyze the leaf quality traits’inheritance characteristics, and provide technical and theoretical basis for germplasm innovation and genetic improvement of mulberry. Two mulberry cross combinations of different ploidy and their parents were used to study the genetic tendency of some leaf quality traits, and main nutritive materials’contents, bioactive materials’contents and anti-oxidation activity weredetermined and analyzed. The results showed that there were great differences among genetic parameters of hybrid progeny group, the inheritance characteristics of water content and ash content were relatively stable. The other quality characteristics, such as solid content, protein content, crude fiber content, phenolic compounds content, flavonoids compounds content, total antioxidant capacity and scavenging hydroxyl radical capacity had great genetic variation. Nutrients contents of hybrid progenies were smaller than those of their parents, while active substances contents and antioxidant activity of hybrid progenies were higher than those of their parents. Higher genetic gain of active substances contents and antioxidant activity should be obtained by selecting superior individuals from those hybrid progenies.