Abstract: To clarify fertilization effects on yield and quality of Biluochun tea cultivated in different intercropping systems, and to provide theoretical and technical support for precision and formula fertilization of Dongting Biluochun tea in Suzhou, a field experiment was conducted to detect 100-bud weight and main nutritional quality of Biluochun tea under different nitrogen rates and the combination of chemical fertilizer with organic manure, taking the tea from 30-year-old pure tea plantations and loquat-tea intercropping in the east Dongting mountain as the sample. The results were as follows: under different rates of nitrogen fertilization, the 100-bud weight reached the heaviest when 450 kg/hm2 nitrogen was applied both in loquattea intercropping and pure tea plantations, with the increase of nitrogen application amount, the 100-bud weight no longer increased and decreased in pure tea garden; meanwhile, the 100-bud weight was the heaviest in loquat-tea intercropping when the ratio of organic to chemical fertilizer was 6:4. Under the same nitrogen rate, the contents of free amino acids, polyphenol and caffeine of Biluochun tea in loquat-tea intercropping were higher than those in pure tea plantation, free amino acids content was positively correlated with nitrogen fertilizer rate. The combined application of organic manure with chemical fertilizer significantly increased the contents of free amino acids and caffeine in tea, and decreased the ratio of tea polyphenols to free amino acids. Furthermore, the amino acids were dramatically increased under such fertilization in loquat-tea intercropping. The caffeine content reached the highest under the treatment of rapeseed cake fertilization at the rate of 450 kg/hm2 both in pure tea plantations and tea- loquat intercropping, and increased by 41.9% and 51.7% respectively in comparison with inorganic nitrogen treatment at the same rate. It is concluded that tea intercropped with loquat and organic fertilization are of great importance to improve the nutritional quality of Biluochun tea, and the optimum nitrogen amount applied to the 30-year-old Biluochun tea plantation is 450 kg/hm2, thus appropriate proportion of organic and inorganic fertilizers can achieve both high yield and high quality of tea.