Abstract: To accurately and objectively evaluate the characteristics of new tested peanut varieties (lines) and promote the extension and application of new peanut varieties in Jiangsu Province, the yielding ability and yield stability of 12 tested peanut varieties from the groups of Huainan and Huaibei were identified by high stability coefficient method. Quality, resistance and the main agronomic characters were also investigated. The results showed that all the tested varieties had above moderate resistance to drought, flood, leaf spot and rust disease. In detail, in Huainan group,‘Ningtai 9922’had relatively high and stable yield, and‘Suhua 0537’ had deep-red seed coat, early maturity, wide adaptability and higher content of crude protein. In Huaibei group, the yields of‘Lian 1001’,‘Ganyu 10-6’,‘Donghua 9’and‘Xu 0627’were 4.7%-7.4% higher than that of‘Xuhua 13’(control) and had higher yield stability. Therefore, these varieties can be used for further demonstration and extension, and high stability coefficient is a feasible method in evaluating the high and stable production quality of peanut varieties (lines).