Abstract: Two years Fuji/malus hupehensis was used to study the effects of different microbial fertilizers on apple growth and absorption, utilization and loss of 15N-urea by 15N isotope labeling. Four treatments were designed: T1 (microbial fertilizer), T2 (microbial fertilizer without carrier), T3 (microbial fertilizer + NPK fertilizers) and CK (no fertilizer). The results showed that the highest total fresh weight, shoot length, root shoot ratio, root activity and root surface area were found in T1, followed by T2, and there was no significant difference between T3 and CK. The differences of soil alkali-hydrolyzable N, available P and available K of different treatments were significant, the highest contents appeared in T1, then was T2, followed by T3, CK was the lowest. In the four treatments, the number of bacteria was the most, then was actinomycetes, and fungi was the least. The highest amount of bacteria, fungi and actinomycetes, and the highest soil enzyme activities, 15N utilization rate and15N residual rate were found in T1, then was T2, no significant difference was found between T3 and CK.