Abstract: Intensive farming produces a large amount of livestock and poultry manure and disorderly emissions, which is one of the important causes of agricultural non-point source pollution. To carry out the effects of livestock manure field disposition on soil properties and fertility is an important research field. Based on two years’ equal fertilizer input - pot experiment to investigate the effects of different fertilizer treatments (manure and chemical fertilizer combined application) on soil nutrients, aggregate fraction and stability in fluvo-aquic soil. The results showed that the available nutrient and organic carbon content of all application fertilizer treatments was higher than that of the control (CK), and it was increased with the application organic fertilizer amount increased. Compared with the only chemical fertilizer application ( T1 ) treatment, soil available N, available P and available K content of combined with pig manure treatment was increased by 3.7%~165.8%、11.6%~197.7%, 20.7%~364.8%, respectively. Compared with the first year experiment soil, the second year experiment soil available N was decreased by 2.8%~48.9%, but available P and available K content was increased. The soil organic carbon was increased by 14.8%~175.7% in all treatments with pig manure addition compared with T1. And the soil aggregates in fluvo-aquic soil were mainly composed of <0.25mm micro-aggregates. Pig manure organic fertilizer combined with chemical fertilizer treatment increased >0.25 mm aggregates content, and the value of MWD, GMD and R0.25, and it increased with application pig manure application amount increased. So, application pig manure organic fertilizer advantageous to increase available nutrients and organic carbon content in fluvo-aquic soil, and promote the macro-aggregates formation and stability.