Abstract: To excavate the excellent tea germplasm resources, 25 wild tea germplasms discovered in Zhenning, Guizhou were taken as materials, the 26 phenotypic traits and 12 major biochemical components were studied by using the statistical, genetic diversity and cluster analysis methods, and the 25 germplasms were evaluated comprehensively. The results showed that these germplasms had the rich variation type in phenotypic traits and main biochemical components. The average variation coefficient of the 26 phenotypic traits was 29.26%, and the average diversity index was 0.87. The average variation coefficient of 12 biochemical components was 25.21%, and the average diversity index was 2.78. The ratio of tea polyphenols to amino acid was between 6.12 and 20.09. These germplasms were divided into three groups by cluster analysis. The tree type of group I was small tree, while group II and III were shrubs. The biochemical components of group III were the most abundant. Three potential excellent germplasm resources were selected, which provided a basis for the protection, development and breeding application of the germplasms in the future.