Abstract: The paper aims to establish a quantitative analysis method for detecting three herbicides’residues in sugarbeet. The authors performed recovery tests by ultra high performance liquid chromatography-tandem mass spectrometry under multiple reaction monitoring mode. The sample was extracted with methanol by accelerated solvent extraction (ASE), and purified by solid phase extraction with methanol, acetone and dichloromethane, 2 mL each, and quantified by the external standard method. The results showed that: the recoveries at three spiked concentration levels of 50, 20, 10 μg/kg ranged from 65.81% to 100.36%, and RSD was between 1.4% and 6.55%, the limits of quantification of this method were in the range of 0.6-2.5 μg/kg. The method is not only fast and simple, but also high sensitive, can satisfy the detection requirement for the three herbicides’residues in sugarbeet simultaneously.