Abstract: The paper aims to explore the delayed effect of different types of organic material after one year mineralization. A pot experiment was carried out with four treatments: (1) without organic material application (TCK); (2) adding maize straw (TS); (3) adding pig manure (TM); (4) adding biochar (TB). Carbon/nitrogen (C/N) of all the four treatments were adjusted as 25:1 in the first year. After a year of mineralization, the major indexes of soil nutrients were measured and analyzed. The results showed that manure and maize straw significantly improved the activity of soil enzymes that related to C and N metabolism. The soil urease activity was significantly enhanced under TM treatment with the maximum value of 2.91 mg/(g·d); while the highest activity of soil sucrose appeared on TS treatment [17.02 mg/(g·d)]. Compared to TCK, the application of organic materials could improve soil nitrogen content after a year of mineralization. Therein, all the forms of soil nitrogen significantly increased under TM. Meanwhile, the content of soil available potassium and organic carbon remarkably increased under the TM and TB treatments with the max value of 1101 mg/kg and 54.56 g/kg, respectively. The max value of soil available phosphorus was found under TM (i.e.727.95 mg/kg). In brief, after a year of mineralization with the same C/N ratio, pig manure and biochar application can significantly improve soil nutrient content in tobacco-planting area of central Henan Province.