Abstract: We aim to investigate the gene and protein properties of GM-CSF. In this study, pUC-GM-CSF was used as a template to amplify GM-CSF gene according to the GM-CSF sequence registered in GenBank, and then this gene was cloned into pMD18-T vector. Sequencing was performed for the recombinant plasmid pT-GM-CSF. Bioinformatics tools were used to analyze homology and ORF of GM-CSF gene. Moreover, the amino acid sequence, hydrophobicity/hydrophilicity, phosphorylation site, subcellular localization and secondary structure were analyzed for the encoded protein. The results showed that the total open reading frame of this gene was 453 bp, encoding 150 amino acids. In the protein encoded by GM-CSF, 4 serine residues and 3 threonine residues may be phosphorylated. The maximum hydrophobicity of GM-CSF protein was 2.267. This protein had 8.91% possibility to be located outside the cell, 0.8% possibility to be located on the cell wall, and 0.24% possibility to be located in cytoplasm. The secondary structure of the protein was mainly composed of random coil, α helix and extension chain. These results can lay a foundation for exploring the immune enhancement mechanism of GM-CSF.