Abstract: The paper aims to achieve efficient facility cultivation of Dendrobium officinale. We designed eight treatments of cultivation in greenhouse with four kinds of cultivation mediums (including granite rubble, purple sandstone gravel, pine bark, pine dust) and three kinds of nutrients (including rinsing, inorganic compound liquid fertilizer, organic liquid fertilizer), to research the effect of different mediums and nutrients on D. officinaleg rowth. The results indicated that the best formula of cultivation medium was surface layer with 4 cm mixture of pine dust and pine bark (1:1, v/v), middle layer with 4 cm purple sandstone gravel, and basement with 2 cm mixture of pine dust and pine bark (1:1, v/v). This cultivation medium formula was ventilated and moisturized, which was good for D. officinale growth and high yield. Meanwhile, to spray suitable concentration of organic liquid fertilizer could significant increase the yield of D. officinale. These results can provide a theoretical basis for achieving high quality and quantity in facility cultivation of D. officinale.