Abstract: The aim is to identify the integrated effect of Crotalaria pallida and Paecilomyces lilacinus against tobacco root- knot nematode disease. The root powders of C. pallida were used as materials to study the influences on the growth, sporulation quantity, spore- germination rate of P. lilacinus, and Meloidogyne incognita J2 corrected death. While three treatments (single intercropping C. pallida, single using P. lilacinus, intercropping C. pallida at the same time using P. lilacinus) were set to make a comprehensive evaluation on integrated C. pallida and P. lilacinus against tobacco root- knot nematode disease. The results showed as follows: (1) the root powders of C. pallida promoted the growth, sporulation quantity, and spore-germination rate of P. lilacinus, under the treatment of 24 h and 0.01 g/mL root powders water extracts of C. pallida and P. lilacinus spore for Meloidogyne incognita J2, the corrected death rate was 96.08%; (2) tobacco intercropping with C. pallida and using P. lilacinus simultaneously could significantly increase the plant height and maximum width of tobacco leaves, the control efficiency was 67.5%, increased by 22.9% and 10.2% compared with single intercropping C. pallida and single using P. lilacinus, respectively. Taken together, C. pallida and P. lilacinus have obvious synergistic effect on controlling tobacco root-knot nematode disease.