Identification and Biocontrol Effect of Growth Promoting Bacteria to Induce Resistance Against Curvularia Leaf Spot in Maize

doi:10.11924/j.issn.1000-6850.casb17110018

Abstract

Abstract: Curvularia leaf spot is a major disease in maize. To screen biocontrol bacteria which efficiently induce resistance against Curvularia leaf spot, four biocontrol bacteria that could induce resistance against Curvularia leaf spot in maize were evaluated with seed coating in pot and field experiments, and the one with best effect on maize growth and yield was also verified. The species identification was performed based upon morphological, physiological and biochemical, and molecular biological methods. The results showed that among the four strains, Sneb2249 exhibited maximum resistance against Curvularia leaf spot, with control effect of 55.04% and 39.36% in pot and field experiments, respectively. Sneb2249 could significantly promote the growth of maize plants: plant height, dry weight of the overground part, and thousand kernel weight were improved by 24.89%, 49.15%, and 7.97%, respectively. Finally, Sneb2249 was identified as Pseudomonas putida. Sneb2249 is an excellent plant growth promoting rhizobacteria that could induce resistance against Curvularia leaf spot and promote the growth and yield of maize. Moreover, our results indicated that seed coating with beneficial microorganisms is a valid plant protection measure for disease control.

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