Abstract: The paper aims to clarify the occurrence regularity of Eotetranychus kankitus (Ehara) and its natural enemies in tea plantations, and provide reference for the prevention and control of the pest mites. A one-year systematic investigation was conducted on Eotetranychus kankitus in Chongqing tea plantation, and the predation function of predator mites was determined. The results showed that the population of E. kankitus was less than that of Polyphagotarsonemus latus in tea garden, but E. kankitus mainly occurred in April to May and caused more serious damage to the tea industry. In tea plantation, there were two predatory mites, Neoseiulus barkeri and Euseius nicholsi, and E. nicholsi was the dominant natural enemy in the peak occurrence period of E. kankitus, and showed an obvious following phenomenon. The result of indoor measurement was that the predation function of E. nicholsi to E. kankitus was the Holling Ⅱ type. The minimum time required for E. nicholsi feeding larva and nymph mite was 1.08 h and the maximum intake was 55 mites per day.