Abstract: The pathogen Phytophthora infestans, which causes potato late blight, delivers effectors inside the living host cells, the effector proteins play an important role for the infection of the pathogen, and are the critical reasons for the pathogen to suppress plant immunity. PITG_RD24 is one of the important effectors of P. infestans which is located in the nucleus of plant cell. To identify the transient expression and resistance of RD24 effector in potato, it is necessary to construct the expression vector of PVX·RD24 (pGR106·RD24). In this study, the fragment of RD24 is inserted to empty vector of pGR106 by the methods of PCR, restriction enzyme digestion and molecular cloning. Then the connected product and plasmid is transformed into DH10B Agrobacterium tumefaciens strains by heat shock method. The expression vector of pGR106 · RD24 is successfully obtained after PCR screening, plasmid extraction, contrasting sequence, and the study can provide the necessary vector for screening potato resistant genes.