Abstract: The paper aims to analyze the growth and development characteristics of grape leaves before and after high temperature occurrence. We analyzed the changes of leaf tissue structure of different grape varieties before and after high temperature occurrence in field. The results showed that: the thickness of leaves, palisade tissue, and spongy tissue of‘Shenfeng’,‘Hupei 1#’,‘Zuijinxiang’,‘Jufeng’,‘Jumeigui’were damaged to various degrees under heat stress; and their CTR decreased and SR increased; the thickness of leaves, palisade tissue and sponge tissue of‘Shenhua’,‘Shenyu’, and‘Summer Black’did not alter obviously, and these 3 varieties showed stronger heat tolerance. CTR and SR did not change significantly before and after high temperature occurrence. Above all, compared with the other 5 varieties,‘Shenhua’,‘Shenyu’, and‘Summer Black’have strong adaptability to high temperature.