Abstract: To provide a basis for the management of kiwifruit orchards, we collected soil samples from the 0-40 cm soil layer, and detected soil nutrients and enzyme activities. The results showed that: (1) the contents of organic matter and available nitrogen were at a low level, while the contents of available phosphorus and available potassium were at a high level; the activities of soil urease, invertase and cellulase increased first and then decreased at 0-10 cm soil layer as the planting age increased, while the activities of these enzymes at 10- 40 cm soil layer had a negative correlation with planting age; the activities of acid phosphorus and catalase decreased with the increase of planting age of kiwifruit orchards; (2) the activities of soil urease, invertase, cellulose, and acid phosphatase decreased with the increase of soil depth, while the activity of soil catalase increased with the increase of soil depth. The results suggest that the soil nutrients and soil enzyme activities are affected by planting age, thus, more organic and nitrogenous fertilizer and less phosphate and potassium fertilizer are needed with the increase of planting age of kiwifruit orchards.