Abstract: The paper aims to explore the effects of perennial grass on soil pH value, organic matter and nutrients by using inter-row grass experiment in kiwifruit orchards in Qinling. 4-year-old‘Hayward’kiwifruit trees were selected in Xi’an kiwifruit test station, inter-row grasses were ryegrass, clover and the combination of ryegrass and clover, at the same time, nature grass was set as control. We determined soil pH value, organic matter, total nitrogen, total phosphorus, total potassium, available phosphorus and available potassium contents in germination stage after 1, 2 and 3 years of inter-row grass, and analyzed the variance. The results showed that: with the increase of the number of cultivation years, total nitrogen and organic matter content had an increasing trend with different grass species; at the third year, available phosphorus and available potassium content decreased with the increase of soil depth; soil pH value of natural grass increased first and then decreased in different soil layers with the increase of the year of inter-row grass; however, soil pH value of clover decreased first and then increased; besides, soil pH value of ryegrass and the combination of ryegrass and clover decreased. Inter-row grass in kiwifruit orchard can improve the soil nutrient status and soil organic matter content, and adjust soil pH value. Different inter-row grass species have different effects on soil pH value. Inter- row of ryegrass and the combination of ryegrass and clover can improve the saline- alkali in northern China.