Genome-wide Identification and Expression Analysis of MLO Gene Family in Capsicum annuum

doi:10.11924/j.issn.1000-6850.casb18020087

Abstract

Abstract: MLO gene is a special kind of negative control factor of disease resistance in plants. The gene mutation causes broad-spectrum resistance of plants. Using bioinformatics, 17 candidate MLO gene sequences were identified in the pepper genome. The range of molecular weight is 187~593kDa, and the range of isoelectric point is 6.65~9.7. Structural analysis showed that the MLO gene of pepper had more introns and had great difference. It was found that the MLO gene of pepper had 20 motifs, and the distribution of the motifs was uniform and the order of arrangement was consistent. Evolutionary analysis showed that the MLO genes of monocotyledon and dicotyledonum were different in evolution, and the closest relationship with capsicum was tomato in the Solanaceae. The expression analysis showed that the MLO genes of Capsicum had a specific expression. The expression of Capana01g003047 was significantly up-regulated by abscisic acid. Capana05g002411 was resistant to abscisic acid and epidemic disease, and was up-regulated under their induction. Capana08g000223 was also up-regulated under the stress of epidemic disease.

References

[1] Devoto A,Piffanelli P,NilssonSI, et al. Topology, subcellular localization, and sequence diversity of the Mlo family inSplants[J] .STheSJournal ofSbiological chemistry,1999 , 274 ( 49 ) : 34993S-35004
[2] Kim M C, Lee S H, Kim J K, et al. Mlo, a modulator of plant defense and cell death, is a novel calmodulin-binding protein. Isolation and characterization of a rice Mlo homologue [J]. Journal of Biological Chemistry, 2002, 277(22): 19304-14.
[3] R Büschges,K Hollricher,R Panstruga,Set al. The barley Mlo gene: a novel control element of plant pathogenSresistance[J]S.Canadian Metallurgical Quarterly,1997,88(5):695-705.
[4] 李凡, 胡东维, 陈锋,等. 小麦TaMlo基因的原核表达、抗体制备及细胞化学分析(简报) [J]. 实验生物学报, 2005, 38(6): 550-4.
[5] 刘建霞, 温日宇, 周凤, 等. 抗白粉病小偃麦衍生系的遗传学研究 [J]. 广东农业科学, 2011, 38(2): 21-2.
[6] ConsonniSC,SHumphry ME,Hartmann HA, et al. Conserved requirement for a plant host cell protein in powdery mildew pathogenesis [J]. Nature genetics, 2006, 38(6): 716-20.
[7] BaiSY,SPavanSS,SZheng Z, et al. Naturally Occurring Broad-Spectrum Powdery Mildew Resistance in a Central American Tomato Accession Is Caused by Loss of Mlo Function [J]. Molecular Plant-Microbe Interactions, 2008, 21(1): 30-9.
[8] PiffanelliSP,SZhouSF,SCasais C, et al. The barley MLO modulator of defense and cell death is responsive to biotic and abiotic stress stimuli [J]. Plant physiology, 2002, 129(3): 1076-85.
[9] SteinSM,SSomerville SC. MLO, a novel modulator of plant defenses and cell death, binds calmodulin [J]. Trends in Plant Science, 2002, 7(9): 379-80.
[10] 闫沛喆, 周索, 李雪辉,等. 全基因组水平上芸薹属Mlo抗病基因的比较分析 [J]. 中国油料作物学报, 2017, 6):
[11] Jones D, Kessler S. Cell type-dependent localization of MLO proteins [J]. Plant Signal Behav, 2017, 12(11): e1393135.
[12] Qin C, Yu C, Shen Y, et al. Whole-genome sequencing of cultivated and wild peppers provides insights into Capsicum domestication and specialization [J]. Proceedings of the National Academy of Sciences, 2014, 111(14): 5135-40.
[13] Kim S, Park M, Yeom S I, et al. Genome sequence of the hot pepper provides insights into the evolution of pungency in Capsicum species [J]. Nature genetics, 2014, 46(3): 270-8.
[14] 魏小春, 姚秋菊, 原玉香,等. 辣椒CaWRKY13基因克隆及非生物胁迫下表达分析 [J]. 分子植物育种, 2016, 10): 2582-8.
[15] Artimo P, Jonnalagedda M, Arnold K, et al. ExPASy: SIB bioinformatics resource portal [J]. Nucleic acids research, 2012, 40(Web Server issue): W597-603.
[16] Hu B, Jin J, Guo A Y, et al. GSDS 2.0: an upgraded gene feature visualization server [J]. Bioinformatics (Oxford, England), 2015, 31(8): 1296-7.
[17] Bailey T L, Boden M, Buske F A, et al. MEME SUITE: tools for motif discovery and searching [J]. Nucleic acids research, 2009, 37(Web Server issue): W202-8.
[18] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method [J]. Methods (San Diego, Calif), 2001, 25(4): 402-8.
[19] 陈玲, 邱显钦, 张颢,等. 不同物种Mlo基因生物信息学分析 [J]. 西南农业学报, 2012, 25(4): 1302-8.
[20] Kim D S, Hwang B K. The pepper MLO gene, CaMLO2, is involved in the susceptibility cell-death response and bacterial and oomycete proliferation [J]. The Plant journal : for cell and molecular biology, 2012, 72(5): 843-55.
[21] Huang S, Weigel D, Beachy R N, et al. A proposed regulatory framework for genome-edited crops [J]. Nature genetics, 2016, 48(2): 109.
[22] Acevedo-Garcia J, Spencer D, Thieron H, et al. mlo-based powdery mildew resistance in hexaploid bread wheat generated by a non-transgenic TILLING approach [J]. Plant Biotechnol J, 2017, 15(3): 367-78.
[23] Chen Z, Hartmann H A, Wu M J, et al. Expression analysis of the AtMLO Gene Family Encoding Plant-Specific Seven-Transmembrane Domain Proteins [J]. Plant Molecular Biology, 2006, 60(4): 583-97.