Abstract: Abstract: In order to further explore and improve the quality of frozen semen and the relative key technique, the quality of 1720 semen samples including dairy cattle, beef cattle (e.g. cattle, yellow cattle, yak, etc) and dairy buffalo, etc. from the domestic bovine frozen semen production enterprises had been analyzed in this research. It showed that the dose value was 0.20 ± 0.008 ml, the motility index was 40.9 ± 2.3%, the progressive motility sperm amount per dose was 1 224.3 ± 2.97 million, the abnormal sperm percentage was 12.6 ± 3.8% and the bacterial count was 38.9 ± 192.7. Motility index showed irregular interannual fluctuation changes, the abnormal sperm percentage in 2016 remarkable decreased by 0.8 and 1.3 respectively, compared with the previous two years. The difference during the three years was significant (P<0.05). The bacterial count in 2014 was significantly higher than that in the other two years (P<0.05). By comparison among breeds, the motility of dairy cattle increased by 0.5% than that of milk buffalo, by 0.2% than beef cattle and cattle (P<0.05). There was no significant difference between milk buffalo, beef cattle and cattle (P > 0.05). The abnormal sperm percentage of milk buffalo increased by 1.9% and 0.9% respectively than that of dairy cattle and beef cattle, and the difference is significant among the breeds (P＜0.05). As for the progressive motility sperm amount per dose, milk buffalo was maximum, dairy cattle was minimum, and the difference among the breeds was significant (P＜0.05). There were distinct differences in the quality index including motility, the progressive motility sperm amount per dose, the abnormal sperm percentage of the samples from different comprises. The above data analysis reflects the general situation of the quality of bovine frozen semen at present in China, and provides the basis for the technical research of frozen semen. Key words: Bulls; frozen semen; quality; evaluation; analysis