Abstract: To explore the scientific application method of bichar, a pot experiment was conducted to compare the effects of biochar, organic manure, and combined application of biochar and organic manure on nutrients, pH value and microorganism of vegetable soil and vegetable growth. The experiment included four treatments, including the control without any application of organic manure and biochar, biochar treatment, combined organic manure+ biochar treatment, and organic manure treatment. As compared with control, the application of biochar increased the fixation of soil organic carbon and decreased soil acidity better than the combined application of organic manure and biochar. While, as to increasing CEC, the contents of water stable aggregates, total N, mineral N, available Fe, available Mn, available Mo, and easily oxidizable organic carbon, micro-biomass C, water soluble organic carbon, and improving soil microflora and soil enzyme activities, the combined application of organic manure and biochar had greater effects than the application of biochar. There was no significant difference in soil bulk density, and the content of available K, P, Ca, Si, Mg, Zn and Cu between the effects of the combined application of organic manure and biochar and the application of biochar. Yield of vegetable under the combined application of organic manure and biochar was slightly higher than that under the single application of biochar. One the whole, the effect of combined application of biochar and organic manure on soil fertility and vegetable growth is better than that of the single application of biochar.