Abstract: The aim is to fully utilize the fine traits of wheat near isogenic lines with three pistils and explore the utility value in wheat breeding. The high molecular weight glutenin, low molecular weight glutenin and gliadin of wheat near isogenic lines with three pistils were analyzed by polyacrylamide gel electrophoresis (PAGE). Taking‘Chinese Spring’(CS) as a reference, cluster analysis was carried out by the band migration distance and band polymorphism of the LMW-GS and gliadin electrophoretic map. The high molecular weight glutenin subunits (HMW-GS) results from sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE) showed that there were 10 types of subunits and 8 types of subunit combination in 10 wheat materials. At the Glu-A1 loci, the frequency of major subunit nulls reached 60%; at the Glu-B1 loci, the frequency of the major subunit 7+8 was 50%; at the Glu-D1 loci, the frequency of the major subunit 2+12 was 60%; the major subunit combination was Null/7+8/2+12. In the low molecular weight glutenin subunits (LMW-GS), 10 wheat materials totally isolated 25 bands, 10 bands were isolated from all materials and the ratio was 50%. The gliadin results from acid polyacrylamide gel electrophoresis (A- PAGE) showed that there were 25 bands isolated from 10 wheat materials totally, 5 bands were isolated from all materials and the ratio was 20%. The results of cluster analysis showed that the genetic distance of 10 wheat materials was 0.03 to 0.28. When the genetic distance was 0.26, 10 materials could be divided into 3 categories. Based on the analysis of glutenin and gliadin and cluster analysis, it can be concluded that wheat near isogenic lines with three pistils can not be used to improve the quality of wheat in breeding, but it can be used to improve the yield of wheat, and the most important task is to improve the 1000-kernel weight.