Abstract: The effects of different explants type, different hormone levels and different sucrose concentration on the growth of callus were studied by tissue culture.so as to establish the callus growth system. Meanwhile,the contents of podophyllotoxin in callus of Sinopodophyllum emodi by HPLC.The results showed that the petiole was optimum explants of callus induction, the best midum Ms+ NAA0.5 mg/L+TDZ 0.1mg/L + 6-BA 1.0mg/L with the sucrose of 40.0 g/L was suitable for callus proliferation. The granular and loose eugenic callus was obtained. The active components podophyllotoxin in callus is up to 1.56%,which is the 68.4% of the components of wild Sinopodophyllum emodi.So, podophyllotoxin can be obtained by culturing the callus of Sinopodophyllum emodi.